A two‐step strategy was used for the detection of the SARS‐CoV‐2 variant B.1.1.7 with initial screening by TaqPath™ RT‐PCR COVID‐19 kit (Thermo Fisher Scientific) followed by whole genome sequencing of spike gene target failure (SGTF) samples. Briefly, viral RNA was extracted using QIAamp Viral RNA Mini kit (Qiagen) according to manufacturer's instructions. ![]() The oropharyngeal swabs of travelers returning from United Kingdom were tested for the presence of SARS‐CoV‐2 through real‐time polymerase chain reaction (PCR) at the Department of Virology, National Institute of Health, Pakistan. We hereby report the detection and genetic characterization of two imported cases of the B.1.1.7 variant in Pakistan. 1, 2 During the current COVID‐19 pandemic, identification of novel lineages of SARS‐CoV‐2 and tracking their geographic spread are essential to guide public health interventions and travel advice. Analysis of 17,782 whole‐genome sequences submitted to GISAID exhibited several mutations mainly in the spike (S) gene. We recommend documenting your own validation with a specific version of Geneious Prime using tools, data and parameters that are as close as possible to what will be used "in production".On December 14, 2020, a variant of SARS‐CoV‐2 characterized as VOC‐202012/01 (lineage B.1.1.7) emerged in the United Kingdom 1 with rapid expansion to over 64 countries as of January 27, 2021. We do not have any specific documents available that would assist you in producing your own validating documentation. See “Advanced Administration” chapter in the Geneious Manual for further information.
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